{"id":10662,"date":"2020-09-08T18:02:49","date_gmt":"2020-09-08T09:02:49","guid":{"rendered":"https:\/\/ebn2.arkray.co.jp\/english\/?page_id=10662"},"modified":"2020-11-24T13:14:44","modified_gmt":"2020-11-24T04:14:44","slug":"glycation-stress_03","status":"publish","type":"page","link":"https:\/\/ebn2.arkray.co.jp\/chinese\/academicinfo\/glycation-stress_03\/","title":{"rendered":"\u7cd6\u5316\u5e94\u6fc0\u6807\u5fd7\u7269\u7684\u68c0\u6d4b\u65b9\u6cd5(2)\u3000AGEs\u7684\u68c0\u6d4b"},"content":{"rendered":"\n

\u7cd6\u5316\u5e94\u6fc0\u6807\u5fd7\u7269\u7684\u68c0\u6d4b\u65b9\u6cd5(2)\u3000AGEs\u7684\u68c0\u6d4b<\/h2>\n\n\n\n

\u540c\u5fd7\u793e\u5927\u5b66\u5927\u5b66\u9662 \u751f\u547d\u533b\u79d1\u5b66\u7814\u7a76\u79d1 \u7cd6\u5316\u5e94\u6fc0\u7814\u7a76\u4e2d\u5fc3
\u516b\u6728\u96c5\u4e4b \u6559\u6388 \u4e3b\u7f16<\/p>\n\n\n\n

\u4f53\u5185\u7684\u8461\u8404\u7cd6\u548c\u86cb\u767d\u8d28\u7684\u975e\u9176\u53cd\u5e94\u8fc7\u7a0b\u4e2d\u4ea7\u751f\u7684\u5404\u79cd\u7269\u8d28\u4e3a\u8bc4\u4ef7\u7cd6\u5316\u5e94\u6fc0\u7684\u6807\u5fd7\u7269\u3002\u672c\u6587\u5c06\u4ecb\u7ecdAGEs\u7684\u68c0\u6d4b\u65b9\u6cd5\u548c\u65e0\u521b\u68c0\u6d4b\u751f\u7269\u4f53\u5185AGEs\u7684\u65b9\u6cd5\u3002<\/p>\n\n\n\n

AGEs\u7684\u68c0\u6d4b<\/h3>\n\n\n

\u5728\u540e\u671f\u53cd\u5e94\u4e2d\u751f\u6210\u7684\u8840\u4e2d AGEs \u5305\u542b\u7fa7\u7532\u57fa\u8d56\u6c28\u9178(N\u03b5<\/sup><\/em>-carboxymethyl lysine ; CML)(1)<\/sup>\u3001\u620a\u7cd6\u7d20(pentosidine)(2)<\/sup>\u3001\u7fa7\u7532\u57fa\u7cbe\u6c28\u9178(N\u03c9<\/sup><\/em>-carboxymethylarginine;CMA)(3)<\/sup>\u7b49\u5404\u79cd\u7269\u8d28\u3002<\/p>\n

CML \u662f\u7531\u8d56\u6c28\u9178\u4ee5 GO(glyoxal)\u4e3a\u4e2d\u95f4\u4f53\u4ea7\u751f\u7684\u4e00\u79cd\u975e\u8367\u5149\u6027\u3001\u975e\u4ea4\u8054\u6027AGEs\uff0c\u5728\u7cd6\u5c3f\u75c5\u548c\u6c27\u5316\u5e94\u6fc0\u52a0\u5267\u65f6\u4e5f\u4f1a\u4ea7\u751f\u3002\u5982\u679c\u5c06\u5df2\u5f62\u6210CML\u7684\u80f6\u539f\u86cb\u767d\u6dfb\u52a0\u5230\u4eba\u76ae\u80a4\u6210\u7ea4\u7ef4\u7ec6\u80de\u7684\u57f9\u517b\u57fa\u4e2d\uff0c\u53ef\u4fc3\u8fdb\u7ec6\u80de\u51cb\u4ea1(4)<\/sup>\u3002\u5728\u76ae\u80a4\u4e2d\uff0c\u5b83\u4e5f\u5b58\u5728\u4e8e\u4ee3\u8c22\u5468\u8f6c\u76f8\u5bf9\u8f83\u5feb\u7684\u8868\u76ae\u5c42(5)<\/sup>\u3002\u89d2\u8d28\u5c42\u4e2d\u7684CML\u84c4\u79ef\u4e0e\u76ae\u80a4\u808c\u7406\u7684\u4e27\u5931\u6709\u5173(6)<\/sup>\u3002<\/p>\n

\u620a\u7cd6\u7d20\u662f\u4e00\u79cd\u7531\u6838\u7cd6\u3001\u7cbe\u6c28\u9178\u3001\u8d56\u6c28\u9178\u9ad8\u6548\u751f\u6210\u7684\u8367\u5149\u6027\u3001\u4ea4\u8054\u6027AGEs\uff0c\u4f5c\u4e3a\u80be\u75c5\u7684\u65e9\u671f\u4e34\u5e8a\u6807\u5fd7\u7269\u4e4b\u4e00\uff0c\u5728\u65e5\u672c\u53ef\u4f7f\u7528\u533b\u4fdd\u3002\u8fd1\u5e74\u6765\uff0c\u8840\u6db2\u548c\u5c3f\u6db2\u4e2d\u7684\u620a\u7cd6\u7d20\u4f5c\u4e3a\u53cd\u6620\u9aa8\u8d28\u8001\u5316\u7684\u6807\u5fd7\u7269\u5907\u53d7\u5173\u6ce8\uff0c\u5e76\u6709\u671b\u7528\u4e8e\u9aa8\u8d28\u758f\u677e\u75c7\u7684\u8bca\u65ad(7)<\/sup>\u3002\u6b64\u5916\uff0c\u620a\u7cd6\u7d20\u8fd8\u5b58\u5728\u4e8e\u76ae\u80a4\u80f6\u539f\u86cb\u767d\u4e2d\uff0c\u5e76\u968f\u5e74\u9f84\u589e\u52a0\u800c\u589e\u591a\u3002\u7cd6\u5c3f\u75c5\u60a3\u8005\u76ae\u80a4\u4e2d\u7684\u620a\u7cd6\u7d20\u84c4\u79ef\u91cf\u9ad8\u4e8e\u540c\u5e74\u9f84\u5065\u5eb7\u4eba(8)<\/sup>\u3002<\/p>\n

CMA \u662f\u4e00\u79cd\u7531\u7cbe\u6c28\u9178\u4ee5 GO \u4e3a\u4e2d\u95f4\u4f53\u4ea7\u751f\u7684AGEs\uff0c\u7279\u5f02\u6027\u5730\u5b58\u5728\u4e8e\u80f6\u539f\u86cb\u767d\u4e2d(3)<\/sup>\u3002<\/p>\n

ELISA\u8bd5\u5242\u76d2\u5df2\u6709\u591a\u5bb6\u516c\u53f8\u5728\u552e\uff0c\u53ef\u7528\u4e8e\u68c0\u6d4b\u8840\u4e2dCML\u548c\u620a\u7cd6\u7d20(9-10)<\/sup>\u3002\u4f46\u662f\uff0c\u68c0\u6d4b\u751f\u7269\u6837\u54c1\u4e2d\u7684 AGEs \u65f6\uff0c\u9700\u8981\u8fdb\u884c\u4e0e\u5f85\u6d4b\u7cd6\u5316\u53cd\u5e94\u4ea7\u7269\u7684\u7279\u6027\u76f8\u7b26\u7684\u5904\u7406\u3002\u68c0\u6d4bCML\u548c\u620a\u7cd6\u7d20\u65f6\uff0c\u5982\u679c\u5c06\u8840\u6db2\u6837\u54c1\u5728\u9ad8\u6e29\u4e0b\u8fdb\u884c\u52a0\u70ed\u5904\u7406\uff0c\u5219\u4f1a\u5728\u6837\u54c1\u9884\u5904\u7406\u8fc7\u7a0b\u4e2d\u4eba\u4e3a\u4ea7\u751f AGEs\uff0c\u5bfc\u81f4\u68c0\u6d4b\u503c\u5347\u9ad8\uff0c\u4ea7\u751f\u8bef\u5dee(11)<\/sup>\u3002<\/p>\n

\u68c0\u6d4b\u620a\u7cd6\u7d20\u65f6\uff0c\u53ef\u91c7\u7528HPLC\u6cd5\u6765\u907f\u514d\u68c0\u6d4b\u503c\u5347\u9ad8\u4ea7\u751f\u8bef\u5dee\u3002\u5728HPLC\u6cd5\u4e2d\uff0c\u5bf9\u8840\u6e05\u6837\u54c1\u8fdb\u884c\u76d0\u9178\u6c34\u89e3\u540e\uff0c\u901a\u8fc7\u79bb\u5b50\u4ea4\u6362\u67f1\u7b49\u9884\u5904\u7406\u9664\u53bb\u6837\u54c1\u4e2d\u7684\u6742\u8d28\uff0c\u518d\u5229\u7528\u53cd\u76f8HPLC\u5206\u79bb\u548c\u5206\u6790\u620a\u7cd6\u7d20(\u56fe\uff11)(12)<\/sup>\u3002\u68c0\u6d4b\u620a\u7cd6\u7d20\u65f6\uff0c\u4f7f\u7528\u620a\u7cd6\u7d20\u7684\u7279\u5f02\u6027\u8367\u5149(\u6fc0\u53d1\u6ce2\u957f 335 nm\uff0c\u53d1\u5c04\u6ce2\u957f 385 nm)\u3002\u4f46\u662f\uff0cHPLC\u6cd5\u5b58\u5728\u8bf8\u5982\u9884\u5904\u7406\u65f6\u7684\u56de\u6536\u7387\u964d\u4f4e\u3001\u907f\u514d\u5728\u5f85\u6d4b\u5cf0\u4e2d\u6df7\u5165\u6742\u8d28\u53ca\u96be\u4ee5\u68c0\u6d4b\u591a\u4e2a\u6837\u54c1\u7b49\u7684\u8bfe\u9898\u3002<\/p>\n

\"\"\u56fe\uff11\u3000\u7528<\/strong>HPLC<\/strong>\u6cd5\u68c0\u6d4b\u8840\u6e05\u620a\u7cd6\u7d20\u7684\u8272\u8c31\u56fe<\/strong>(12)
<\/sup><\/strong>\u8272\u8c31\u67f1:Vydac 218TP54(250 x 4.6mm ID,5 \u00b5m)
\u6d17\u8131\u6db2:0.1% HFBA \/ acetonitrile(82.5 \/ 17.5), \u6d41\u901f:1.0 mL\/min,
\u6e29\u5ea6:30\u2103,\u68c0\u6d4b\u51fa\uff1a\u8367\u5149(ex 330 nm \/ em 375 nm)<\/h6>\n\n\n

\u5229\u7528AGE Reader\u00ae<\/sup>\u65e0\u521b\u68c0\u6d4b\u76ae\u80a4\u4e2d\u7684AGEs<\/h3>\n\n\n

\u76ae\u80a4\u4e2d\u7684AGEs\u84c4\u79ef\u88ab\u8ba4\u4e3a\u662f\u76ae\u80a4\u7d27\u81f4\u5ea6\u548c\u5f39\u6027\u964d\u4f4e\u7b49\u76ae\u80a4\u8001\u5316\u7684\u56e0\u7d20\u4e4b\u4e00\u3002\u53ef\u4ee5\u5229\u7528AGE Reader(DiagnOptics\u516c\u53f8\u5236)\u68c0\u6d4b\u76ae\u80a4\u7684\u8367\u5149\u5f3a\u5ea6(auto fluorescence; AF)\uff0c\u7531\u6b64\u65e0\u521b\u68c0\u6d4b\u76ae\u80a4\u7684\u8367\u5149\u6027AGEs\u84c4\u79ef\u91cf(13-14)<\/sup>\u3002<\/p>\n

AGE Reader \u662f\u4e3a\u8bc4\u4ef7\u7cd6\u5c3f\u75c5\u5e76\u53d1\u75c7\u8fdb\u5c55\u98ce\u9669\u800c\u7814\u53d1\u7684\u4e00\u79cd\u65e0\u521b\u68c0\u6d4b\u76ae\u80a4\u4e2dAGEs\u84c4\u79ef\u91cf\u7684\u4e34\u5e8a\u68c0\u9a8c\u4eea\u5668(15-17)<\/sup>\u3002\u5f53\u76ae\u80a4\u53d7\u5230\u7d2b\u5916\u7ebf\u7167\u5c04\u65f6\uff0c\u84c4\u79ef\u5728\u7ec4\u7ec7\u5185\u7684\u8367\u5149\u6027AGEs \u88ab\u6fc0\u53d1\u5e76\u53d1\u51fa\u7279\u6709\u7684\u81ea\u53d1\u8367\u5149\uff0c\u672c\u4eea\u5668\u6b63\u662f\u5229\u7528\u8fd9\u4e00\u6027\u8d28\u68c0\u6d4bAGEs(\u56fe2)\u3002<\/p>\n

\"\"<\/p>\n

\u56fe2\u3000AGE Reader\u00ae<\/sup>\u7684\u68c0\u6d4b\u7cfb\u7edf\u548c\u4e3b\u673a\u7ed3\u6784\u6a21\u5f0f\u56fe
<\/strong>A : \u624b\u81c2(\u68c0\u6d4b\u90e8\u4f4d)\uff0cB : \u5149\u6e90(Black light)\uff0cC : \u73bb\u7483\u7ea4\u7ef4\uff0cD : \u5206\u5149\u955c<\/h6>\n

\u5728AGE Reader \u7684\u68c0\u6d4b\u7cfb\u7edf\u4e2d\uff0c\u5c06\u7531\u8bbe\u7f6e\u5728\u76d2\u5f62(280(\u957f)\u00d7150(\u5bbd)\u00d7115(\u9ad8)mm)\u4e3b\u673a\u5185\u7684\u5149\u6e90(UV-A black-light)\u53d1\u51fa345\uff5e410 nm(\u6700\u5927\u6ce2\u957f365 nm)\u6ce2\u957f\u7684\u5149\uff0c\u901a\u8fc7\u7ea62\u00d72 cm \u7684\u73bb\u7483\u7a97\u4f5c\u4e3a\u6fc0\u53d1\u5149\u7167\u5c04\u5230\u76ae\u80a4\u8868\u9762\u3002\u672c\u4eea\u5668\u5c06\u6fc0\u53d1\u5149\u7ecf\u76ae\u80a4\u53d1\u51fa\u7684 420\uff5e600 nm \u7684\u8367\u5149\u901a\u8fc7\u73bb\u7483\u7ea4\u7ef4\u5f15\u5165\u5206\u5149\u955c\uff0c\u5229\u7528\u4e13\u4e1a\u8f6f\u4ef6\u5206\u6790\u6240\u5f97\u4fe1\u606f\u540e\uff0c\u8ba1\u7b97 AF(18)<\/sup>\u3002\u68c0\u6d4b\u65f6\u95f4\u7ea6\u4e3a90\u79d2(3\u6b21\u68c0\u6d4b\u6a21\u5f0f\u7684\u60c5\u51b5\u4e0b)\u3002AGE Reader \u8fd8\u53ef\u4ee5\u5728\u68c0\u6d4b\u8367\u5149\u7684\u540c\u65f6\uff0c\u68c0\u6d4b\u767d\u8272LED\u5149\u7684\u76ae\u80a4\u53cd\u5c04\u5149(reflection ; Ref)\uff0c\u5e76\u5728Ref\u4f4e\u4e8e12\uff05\u65f6\u6821\u6b63\u68c0\u6d4b\u503c\u3002\u4f46\u662f\uff0c\u5728Ref\u4f4e\u4e8e6\uff05\u65f6\u65e0\u6cd5\u68c0\u6d4b\u3002\u56e0\u6b64\uff0c\u65e0\u6cd5\u68c0\u6d4b\u80a4\u8272\u4e3a\u6df1\u68d5\u8272\u3001\u80a4\u8272\u8f83\u9ed1\u7684\u53d7\u8bd5\u8005(Fitzpatrick class 5-6 skin color)\u3002\u53e6\u5916\uff0c\u68c0\u6d4b\u503c\u4f1a\u53d7\u4f7f\u7528\u542b\u9632\u6652\u971c\u7b49\u5728\u5185\u7684\u62a4\u80a4\u5316\u5986\u54c1\u7b49\u7684\u5f71\u54cd\uff0c\u56e0\u6b64\u68c0\u6d4b\u524d\u9700\u5f7b\u5e95\u6e05\u6d17\u68c0\u6d4b\u90e8\u4f4d\u3002<\/p>\n

\u76ae\u80a4AF\u53ef\u6839\u636e\u8367\u5149(420\uff5e600 nm)\u5f3a\u5ea6\u5360\u6fc0\u53d1\u5149(300\uff5e420 nm)\u7684\u6bd4\u4f8b\u8ba1\u7b97\u51fa\u6765\uff0c\u5e76\u53ef\u7528\u4efb\u610f\u5355\u4f4d(arbitrary units;AU)\u8868\u793a\u3002\u7531AGE Reader\u8ba1\u7b97\u51fa\u6765\u7684 AF \u5c06\u7efc\u5408\u8003\u8651\u53d7\u8bd5\u8005\u7684\u6027\u522b\u3001\u5e74\u9f84\u7b49\uff0c\u4e0e\u751f\u4ea7\u5546\u6240\u79ef\u7d2f\u6570\u636e\u7684\u6bd4\u8f83\u56fe\u8868\u4e00\u540c\u8f93\u51fa\u3002\u4f46\u662f\uff0c\u751f\u4ea7\u5546\u5c1a\u672a\u516c\u5f00AF\u7684\u5206\u6790\u516c\u5f0f\u3002<\/p>\n

\u4f7f\u7528AGE Reader\u68c0\u6d4b\u7684AF\u503c\u663e\u793a\u4e86\u80a4\u8272\u767d\u7684\u4eba\u7fa4(Fitzpatrick class 1-3 skin color)\u4e2d\u5728\u7cd6\u5c3f\u75c5\u60a3\u8005\u548c\u5065\u5eb7\u4eba\u91cc\u4e0e\u5e74\u9f84\u589e\u957f\u7684\u5173\u7cfb\u3002\u68c0\u6d4b\u503c\u968f\u5e74\u9f84\u589e\u957f\u548c\u7cd6\u5c3f\u75c5\u800c\u5347\u9ad8\u3002<\/p>\n

\u751f\u4ea7\u5546\u5efa\u8bae\u7684\u76ae\u80a4AGEs\u68c0\u6d4b\u90e8\u4f4d\u662f\u524d\u81c2\u3002\u68c0\u6d4b\u65f6\uff0c\u524d\u81c2\u7684\u68c0\u6d4b\u5bf9\u53d7\u8bd5\u8005\u7684\u52a8\u4f5c\u8d1f\u62c5\u8f83\u5c0f\uff0c\u4f46\u4e0d\u540c\u5b63\u8282\u660e\u663e\u5f71\u54cd\u53d7\u8bd5\u8005\u53d7\u7d2b\u5916\u7ebf\u7167\u5c04\u7684\u7a0b\u5ea6\u3002\u4ee5\u80a4\u8272\u767d\u7684\u4eba\u7fa4\u4e3a\u5bf9\u8c61\u8fdb\u884c\u68c0\u6d4b\u90e8\u4f4d\u6bd4\u8f83\u7684\u7ed3\u679c\u663e\u793a\uff0c\u524d\u81c2\u548c\u4e0b\u80a2\u4e0e\u7cd6\u5c3f\u75c5\u5e76\u53d1\u75c7\u5bc6\u5207\u76f8\u5173(19)<\/sup>\u3002<\/p>\n

\u53e6\u4e00\u65b9\u9762\uff0c\u65e5\u672c\u4eba\u7684\u76ae\u80a4\u66b4\u9732\u5728\u7d2b\u5916\u7ebf\u4e0b\u65f6\u4f1a\u53d8\u7ea2\uff0c\u80a4\u8272\u4f1a\u53d8\u6df1\u3002\u56e0\u6b64\uff0c\u5728\u68c0\u6d4b\u65e5\u672c\u4eba\u65f6\uff0c\u5bf9\u4e0d\u6613\u53d7\u7d2b\u5916\u7ebf\u5f71\u54cd\u7684\u76ae\u80a4\u6d4b\u91cf\u68c0\u67e5\u4e2d\u63a8\u8350\u7684\u4e0a\u81c2\u5185\u4fa7(\u5bf9\u8ddd\u53f3\u81c2\u8098\u7aef\u7ea610cm\u90e8\u4f4d)\u53ef\u4ee5\u7a33\u5b9a\u68c0\u6d4b(\u56fe\uff13)(20-21)<\/sup>\u3002\u4f7f\u7528AGE Reader\u7684\u76ae\u80a4AGEs\u68c0\u6d4b\u90e8\u4f4d\u9700\u6839\u636e\u7d2b\u5916\u7ebf\u7684\u5f71\u54cd\u3001\u80a4\u8272\u3001\u5f85\u6d4b\u4eba\u79cd\u9002\u5f53\u9009\u62e9\u3002<\/p>\n

\"\"<\/p>\n

\u56fe<\/strong>3 <\/strong>\u4f7f\u7528<\/strong>AGE Reader \u00ae<\/strong>\u68c0\u6d4b\u4e0a\u81c2\u5185\u4fa7
<\/strong>\u7ea2\u8272\u7bad\u5934\u8868\u793a\u68c0\u6d4b\u90e8\u4f4d<\/h6>\n\n\n

\u91c7\u7528\u80f6\u5e26\u7c98\u8d34\u6cd5\u68c0\u6d4b\u89d2\u8d28\u5c42CML<\/h3>\n\n\n

\u89d2\u8d28\u5c42\u4f4d\u4e8e\u76ae\u80a4\u6700\u5916\u5c42\uff0c\u7531\u89d2\u8d28\u7ec6\u80de\u548c\u7ec6\u80de\u95f4\u8d28\u7ec4\u6210\u3002\u89d2\u8d28\u5c42\u56e0\u8868\u76ae\u5c42\u4e0d\u65ad\u4ea7\u751f\u7684\u89d2\u8d28\u7ec6\u80de\u548c\u4ece\u8868\u9762\u7684\u5265\u8131\u800c\u4e0d\u65ad\u91cd\u5efa\uff0c\u7ef4\u6301\u673a\u4f53\u9632\u5fa1\u529f\u80fd\uff0c\u5982\u4fdd\u6e7f\u3001\u5c4f\u969c\u529f\u80fd\u7b49\u3002\u53e6\u4e00\u65b9\u9762\uff0c\u6709\u5173\u5e74\u9f84\u589e\u957f\u5f15\u8d77\u76ae\u80a4\u53d8\u5316\u7684\u7814\u7a76\u591a\u662f\u4ece\u7ec4\u7ec7\u5b66\u7684\u89d2\u5ea6\u8fdb\u884c\u7684\uff0c\u80fd\u4ece\u751f\u5316\u89d2\u5ea6\u8fdb\u884c\u7b80\u5355\u68c0\u6d4b\u7684\u65b9\u6cd5\u8f83\u5c11\u3002<\/p>\n

\u4f17\u6240\u5468\u77e5\uff0c\u80f6\u5e26\u7c98\u8d34\u6cd5\u4f7f\u7528\u80f6\u5e26\u5265\u79bb\u91c7\u96c6\u89d2\u8d28\u5c42\u7ec6\u80de\uff0c\u662f\u4e00\u79cd\u80fd\u65e0\u521b\u8bc4\u4ef7\u89d2\u8d28\u5c42\u529f\u80fd\u7684\u65b9\u6cd5\u3002\u5728\u80f6\u5e26\u7c98\u8d34\u6cd5\u4e2d\uff0c\u62a5\u544a\u4e86\u8131\u843d\u89d2\u8d28\u7ec6\u80de\u6570\u3001\u89d2\u8d28\u7ec6\u80de\u9762\u79ef\u3001\u7ec6\u80de\u5f62\u6001\u548c\u7ec6\u80de\u6838\u5b58\u6d3b\u7387\u68c0\u6d4b\u7b49\u5f62\u6001\u5b66\u7684\u8bc4\u4ef7\u65b9\u6cd5\u3002<\/p>\n

\u53e6\u4e00\u65b9\u9762\uff0c\u8fd8\u62a5\u544a\u4e86\u7528\u80f6\u5e26\u7c98\u8d34\u6cd5\u5265\u79bb\u91c7\u96c6\u89d2\u8d28\u5c42\u4e2d\u7684\u86cb\u767d\u8d28\u3001\u89d2\u86cb\u767d(22)<\/sup>\u3001\u7ec4\u7ec7\u86cb\u767d\u9176(23)<\/sup>\u3001\u8f6c\u8c37\u6c28\u9170\u80fa\u9176(24)<\/sup>\u3001\u7cbe\u6c28\u9178\u9176(25)<\/sup>\u7b49\u751f\u5316\u68c0\u6d4b\u3002<\/p>\n

\u91c7\u7528\u80f6\u5e26\u7c98\u8d34\u6cd5\u68c0\u6d4b\u89d2\u8d28\u5c42CML\uff0c\u53ef\u4ee5\u65b9\u4fbf\u91c7\u96c6\u5e76\u76f4\u63a5\u68c0\u6d4b\u76ae\u80a4\u86cb\u767d\u4e2d\u7684\u7cd6\u5316\u5e94\u6fc0\u72b6\u6001(26)<\/sup>\u3002\u5c06\u5e02\u552e\u7c98\u5408\u7247\uff0c\u5982\u89d2\u8d28\u68c0\u67e5 AST-01(ASAHI BIOMED\u516c\u53f8\u5236)\u7b49\uff0c\u7528\u624b\u6307\u6309\u538b\u5728\u53d7\u8bd5\u8005\u53f3\u4e0a\u81c2\u5185\u4fa7\u7ea6 5\u79d2\uff0c\u7136\u540e\u4ece\u76ae\u80a4\u4e0a\u5265\u79bb\uff0c\u91c7\u96c6\u89d2\u8d28\u5c42(\u56fe\uff14)\u3002\u5c06\u91c7\u96c6\u6709\u89d2\u8d28\u5c42\u7684\u7c98\u5408\u7247\u5207\u788e\u540e\uff0c\u5728Tris-HCl \u7f13\u51b2\u6db2(pH 7.5)\u4e2d\u4f7f\u7528\u5fae\u578b\u5300\u6d46\u5668\u7b49\u5300\u6d46\uff0c\u4ee5\u4fbf\u5c06\u7c98\u5408\u7247\u8868\u9762\u7684\u7c98\u5408\u5242\u522e\u6389\uff0c\u7136\u540e\u56de\u6536\u89d2\u8d28\u5c42\u63d0\u53d6\u6db2\u3002\u4f7f\u7528\u5e02\u552e\u7684\u7528ELISA\u6cd5\u6765\u68c0\u6d4b\u7684\u8bd5\u5242\u76d2\uff0c\u6765\u68c0\u6d4b\u89d2\u8d28\u5c42\u63d0\u53d6\u6db2\u4e2d\u7684 CML\u3002\u540c\u65f6\u53e6\u884c\u68c0\u6d4b\u89d2\u8d28\u5c42\u86cb\u767d\u63d0\u53d6\u6db2\u4e2d\u7684\u86cb\u767d\u91cf\uff0c\u8ba1\u7b97\u51fa\u6bcf\u79cd\u89d2\u8d28\u5c42\u86cb\u767d\u7684 CML\u91cf\u3002<\/p>\n

\"\"<\/p>\n

\u56fe\uff14\u3000\u89d2\u8d28\u5c42\u80f6\u5e26\u7c98\u8d34\u6cd5
<\/strong>\u4e0a\u81c2\u5185\u4fa7<\/h6>\n
\n
References<\/h5>\n
    \n
  1. \n
      \n
    1. Nagai R, et al.<\/i> : Biochem Biophys Res Commun. 1997; 234: 167-172<\/li>\n
    2. Sell DR, et al.<\/i> : J Biol Chem. 1989; 264: 21597-21602<\/li>\n
    3. Iijima K, et al.<\/i> : Biochem J. 2000; 347: 23-27.<\/li>\n
    4. Alikhani Z, et al.<\/i> : J Biol Chem. 2005; 280: 12087-12095.<\/li>\n
    5. Kawabata K, et al.<\/i> : Biochimica et Biophysica Acta. 2011; 1814: 1246?1252.<\/li>\n
    6. \u4e94\u5473\u8cb4\u512a : BIO INDUSTRY. 2011; 28: 20-26.<\/li>\n
    7. Saito M, et al.<\/i> : Osteoporos Int. 2006; 17: 986-995.<\/li>\n
    8. Dyer DG, et al.<\/i> : J Clin Invest. 1993; 91: 2463-2469.<\/li>\n
    9. Tahara N, et al.<\/i> : Cardiovascular Therapeutics. 2012; 30: 42?48.<\/li>\n
    10. Sanaka T, et al.<\/i> : Nephron. 2002; 91: 64-73.<\/li>\n
    11. Nakano M, et al.<\/i> : Anti-Aging Medicine. 2010; 7: 92-93.<\/li>\n
    12. Slowik-Zylka D, et al.<\/i> : J Biochem Biophys Methods. 2004; 61: 313-329.<\/li>\n
    13. Meerwaldt R, et al.<\/i> : Diabetologia. 2004; 47: 1324-1330.<\/li>\n
    14. Meerwaldt R, et al.<\/i> : J Am Soc Nephrol. 2005; 16: 3687-3693.<\/li>\n
    15. Meerwaldt R, et al.<\/i> : J Am Soc Nephrol. 2005; 16: 3687-3693.<\/li>\n
    16. Lutgers HL, et al.<\/i> : Diabetes Care. 2006; 29: 2654-2659.<\/li>\n
    17. Nomoto K, et al.<\/i> : Anti-Aging Medicine. 2010; 7: 73-84.<\/li>\n
    18. AGE Reader User manual Ver2.3, DiagnOptics, pp29.<\/li>\n
    19. Tanaka K, et al.<\/i> : Ther Apher Dial. 2010; 14: 334-340.<\/li>\n
    20. Nomoto K, et al.<\/i> : Anti-Aging Medicine. 2012; 9: 165-173.<\/li>\n
    21. Miyazaki R et al.<\/i> : Anti-Aging Medicine. 2009; 6: 83-94.<\/li>\n
    22. \u516c\u958b\u7279\u8a31\u516c\u5831(A), \u7279\u958b2008-249429.<\/li>\n
    23. \u7530\u539f\u7950\u52a9\u3089\uff1aBUNSEKI KAGAKU. 2009; 58: 15-19.<\/li>\n
    24. \u516c\u958b\u7279\u8a31\u516c\u5831(A), \u7279\u958b2001-29099.<\/li>\n
    25. \u516c\u958b\u7279\u8a31\u516c\u5831(A), \u7279\u958b2010-151482.<\/li>\n
    26. Kamitani Y, et al.<\/i> : Anti-Aging Medicine. 2013; 10: 55-59.<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<\/div>\n\n\n
      \n\n\n\n
      \u7cd6\u5316\u5e94\u6fc0\u548c\u6297\u8870\u8001<\/strong><\/h5>\n\n\n\n